 | Milla Jovovich | as | Alice |
 | Sienna Guillory | as | Jill Valentine |
 | Oded Fehr | as | Carlos Olivera |
 | Thomas Kretschmann | as | Major Cain |
 | Sophie Vavasseur | as | Angie Ashford |
 | Razaaq Adoti | as | Sgt. Peyton Wells (as Raz Adoti) |
 | Jared Harris | as | Dr. Ashford |
 | Mike Epps | as | L.J. |
 | Sandrine Holt | as | Terri Morales |
 | Matthew G. Taylor | as | Nemesis |
 | Zack Ward | as | Nicholai Ginovaeff |
 | Iain Glen | as | Dr. Isaacs |
 | Dave Nichols | as | Captain Henderson |
 | Stefan Hayes | as | Yuri Loginova |
 | Geoffrey Pounsett | as | Mackenzie |
 | Shaun Austin-Olsen | as | Priest |
| Geny Walters | as | Mrs. Priest | |
 | Megan Fahlenbock | as | Marla Mapes |
| Robert Morelli | as | Director | |
 | Aaron Abrams | as | Assistant |
 | Chris Benson | as | Lance Halloran |
| Martin Martinuzzi | as | Overworked Detective | |
 | Brad Borbridge | as | Overworked Sergeant |
 | Frank Chiesurin | as | Sniper |
| Ron Benjamin | as | Bum | |
 | Kevin Hare | as | Home Owner / Scientist |
 | Heather Dick | as | Older Woman |
 | Alex Courey | as | Suit #1 |
 | John Detoro | as | Suit #2 |
 | Billy Khoury | as | Suit #3 |
 | Sava Drayton | as | Suit #4 |
 | Nicole Ann St. Hill | as | Undead Gaudy Hooker |
 | Ricardo Betancourt | as | Burly Cop #1 |
 | Ted Ludzik | as | Burly Cop #2 |
 | Michelle Latimer | as | Tech #1 |
 | Catherine Burdon | as | Tech #2 |
| Rebecca Sims | as | Undead Little Girl | |
| Christopher Weedon | as | Undead Little Boy #1 | |
 | Billy Parrott | as | Security Guard |
 | Derek Aasland | as | Featured Graveyard Undead 1 (as Derek Asland) / Featured "Surplus" Undead #3 |
 | Richard Harte | as | Featured Graveyard Undead 2 / Featured Rooftop Undead #2 |
| Simon Girard | as | Chopper Pilot #1 | |
 | Dean Copkov | as | Chopper Pilot #2 |
 | Jeff White | as | Chopper Pilot #4 |
 | Tim Burd | as | Refugee #1 |
| Natalie Charles | as | Refugee #2 | |
| Pierre Henry | as | Screaming Soldier | |
 | Jim Codrington | as | Lieutenant |
 | Deborah Valente | as | Tech |
| Malcolm Fiddick | as | Reflection Undead | |
| Malcolm Xerxes | as | Featured City Hall Undead #1 | |
 | Michy Gustavia | as | Featured City Hall Undead #2 |
 | Shane Mackinnon | as | Featured City Hall Undead #3 |
 | Ed Fielding | as | Featured City Hall Undead #4 |
 | Stephen R. Hart | as | Featured City Hall Undead #5 (as Stephen Hart) |
 | Sandi Stahlbrand | as | Reporter #1 |
 | Christopher Marren | as | Guard (as Chris Marren) |
 | Tom Gerhardt | as | Zombie Dad |
 | Amber Marshall | as | Daughter |
 | Venice Grant | as | Undead Stripper #1 |
 | Ana Danilina | as | Undead Stripper #2 |
 | Ingrid Johansson | as | Featured IV Woman Undead |
| Jeffrey Weedon | as | Undead Little Boy #2 | |
 | Melanie Tonello | as | Featured Undead Kid #1 |
 | Annabel Chu | as | Featured Undead Kid #2 |
| Joey Jefferson | as | Featured Undead Kid #3 | |
| Heather Braaten | as | Featured Rooftop Undead #1 | |
 | Joey Beck | as | Featured Rooftop Undead #3 |
| Eric Morgan | as | Featured Undead City Walker #1 | |
 | Gene Pyrz | as | Featured Undead City Walker #2 |
| Torquil Colbo | as | Featured Undead City Walker #3 | |
| Joan Moore | as | Featured Undead City Walker #4 | |
| Peter C. Wylde | as | Featured Undead City Walker #5 | |
| Garry Geddes | as | Featured Undead City Walker #6 | |
| Dionne Renee | as | Featured Undead City Walker #7 | |
 | Janessa Crimi | as | Featured Undead Kid #4 |
 | Dan Ellery | as | Featured Graveyard Undead 1 (as Derek Asland) / Featured "Surplus" Undead #3 |
 | Sharon Moore | as | Featured "Surplus" Undead #2 |
 | Jo Chim | as | Network Anchor #1 |
| Bobby Prochaska | as | Network Anchor #2 | |
 | Jazz Mann | as | Reporter #2 |
 | Larissa Gomes | as | Reporter #3 |
 | Gregory White | as | Lab Technician |
 | Roman Podhora | as | Leader Umbrella Guard |
 | Brian King | as | Umbrella Gate Guard |
| Rob Hall | as | Featured School Undead | |
 | Eric Mabius | as | Matt Addison (archive footage) |
